Aria 8.0 manuales

Aria 8.0 Manual de usuario (16 paginas)

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Flow Cytometry Core Facility

1

Prepared by Marc Barnard

1

Table of Contents

1

Introduction

2

Starting up the system

4

Optimizing the side streams

6

Aiming the streams

7

Page 10

10

Page 11

11

Page 12

12

Page 13

13

Page 14

14

Page 15

16

Aria 8.0 Manual de usuario (23 paginas)

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Manual de utilizare

1

Evolio Aria 8.0

1

Măsuri de protecţie

2

Cuprins

5

Accesorii

6

Butoane şi porturi

6

Încărcarea bateriei

7

Pornirea unităţii

8

Ecranul de start

8

Aplicaţii preinstalate

9

Deblocarea ecranului

10

Accesul la Internet

10

Lansarea browserului de Web

11

Biblioteca de fişiere audio

12

Vizualizarea fotografiilor

15

Navigarea în fişiere

16

Setarea contului de email

18

Gestionarea conturilor

19

Ieşirea HDMI

20

Gestionarea operaţiunilor

21

Depanare

22

Specificaţii

22

Aria 8.0 Guía de usuario (344 paginas)

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BD FACSAria II

1

User’s Guide

1

FCC Information

3

Contents

5

About This Guide

13

Conventions

14

Technical Assistance

15

Limitations

16

Cytometer Components

17

Fluidics Cart

18

Power and Operation

21

Flow Cytometer

22

Fluidics Components

23

Sample Injection Chamber

25

Tube Holders

26

Cuvette Flow Cell

27

Figure 1-11 Nozzle

28

Sort Block

29

Deflection Plates

30

Aspirator Drawer

30

Aerosol Management

31

Sort Collection Chamber

32

Optics System

33

Collection Optics

35

Detectors

36

Stream-Viewing Optics

38

Cytometer Electronics

39

Emergency Stop Button

40

Workstation

41

Theory of Operation

43

Fluid Movement

44

Sample Flow

46

Hydrodynamic Focusing

47

Signal Generation

48

Fluorescent Signals

49

Signal Detection

50

Longpass Filters

52

Bandpass Filters

53

% transmission

54

BP 500/50 filter

54

DF 500/50 filter

54

Neutral Density Filters

55

Electronic Processing

57

Pulse Parameters

58

Laser Delay

59

Drop Formation

61

Breakoff Window

62

Side Stream Formation

65

Drop Delay Overview

67

Drop Charging

68

Yield Mask

70

Purity Mask

71

Phase Mask

72

Sort Precision Modes

73

Defining New Precision Modes

75

Using BD FACSDiva Software

77

Workspace Components

78

Cytometer Controls

79

Change Sample Filter

80

Cleaning Modes

80

Sheath Pressure

80

Sample Agitation

81

Sample Temperature

82

Fluidics Level Indicators

83

Cytometer Configuration

84

Cytometer Status Report

88

Custom Configurations

90

Copying a Base Configuration

92

Select the Sort Setup that

95

Configuration Mismatch Dialog

96

Acquisition Controls

97

Sorting Controls

98

Sort Menu

100

Sort Setup

101

Sort Layout

103

Setting Up a Sort Layout

105

Editing a Sort Layout

107

Using Sorting Controls

107

Using Counters

108

Monitoring a Sort

109

Sort Report

110

Templates

112

Running Samples

113

Cytometer Startup

114

Performing Fluidics Startup

115

Starting the Stream

118

Setting Up the Breakoff

119

Setting Up the Fluidics Cart

122

Refilling the Sheath Tank

123

ATTENTION

124

Emptying the Waste Container

127

Waste (A)

129

Preparing the CS&T Beads

133

Running a Performance Check

134

Reviewing the Results

134

Application Settings

136

Adjusting Area Scaling

137

Figure 4-14 FSC and SSC Plot

138

Optimizing PMT Voltages

141

Saving Application Settings

143

Data Collection

144

Calculating Compensation

148

Data Recording and Analysis

151

Setting Up the Experiment

152

Recording Data

155

Analyzing Data

156

Performing a Batch Analysis

159

Setting Up for Sorting

162

Setting Up for Bulk Sorting

164

Using Manual Drop Delay

168

Overview of Auto Drop Delay

171

Using Auto Drop Delay

171

Pausing and Resuming a Sort

178

Setting Up the Stream

181

Test Sort button

182

Creating a Custom Device

184

Deleting a Custom Device

186

Shutdown and Maintenance

187

Daily Shutdown

188

Fluidics Shutdown

190

External Cleaning

193

Scheduled Maintenance

194

Sample Line Backflush

195

Prime After Tank Refill

196

Prepare for Aseptic Sort

197

DI water port

198

DI water line

198

Purging the Fluid Filters

200

Purging the Sheath Filter

200

Changing Fluid Filters

202

Changing the Sheath Filter

203

Changing the Sample Lines

204

Figure 6-12 Ferrule tool

207

Pinch Valve End

208

Flow Cell End

209

Changing the Air Filters

211

Unscheduled Maintenance

213

Changing Nozzles

214

Cleaning the Nozzle

216

Cleaning the Camera Windows

225

Upper Camera Window

226

Apply lubricant to outside

229

Using Custom Optical Filters

230

Cleaning the Optical Filters

231

FSC ND filter

232

Set screw

232

Nozzle holder

232

Troubleshooting

233

Troubleshooting the Stream

234

Troubleshooting the Breakoff

238

Sorting Troubleshooting

239

Acquisition Troubleshooting

244

Fluidics Troubleshooting

251

Electronics Troubleshooting

252

Technical Specifications

253

Cytometer Specifications

254

Performance

255

Sort Performance

256

Excitation Optics

257

Emission Optics

257

Detector Arrays

258

Fluidics Cart Specifications

259

Appendix A

261

Supplies and Consumables

261

Cytometer Supplies

262

Accessory Kit

264

Other Replacement Parts

266

Consumables

267

Reagents

268

Appendix B

271

Near UV Laser Option

271

System Laser Configurations

272

Specifications

273

Operation

274

Instrument Setup

278

Experiment Setup Tips

278

Appendix C

281

BD Aerosol Management Option

281

Option Components

282

ULPA Filter

283

Starting Up the Evacuator

284

Maintenance

289

Replacing the ULPA Filter

290

Reset button

293

Replacing the Air Filter

294

Appendix D

301

Temperature Control Option

301

Setting Up the Water Bath

303

Cooling OutCooling In

304

Setting Up the Tube Holder

305

Input tubing

306

Output tubing

306

Setting Up the ACDU Stage

307

Starting Up the Water Bath

309

Appendix E

313

QC Using BD FACSDiva Software

313

Software

314

Preparing QC Particles

316

Setting Up the QC Experiment

317

Recording QC Data

325

Figure E-6 Recorded QC data

327

Reusing the QC Experiment

328

Tracking QC Results

329

Numerics

331

Aria dispositivos